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US20060228334A1 Modified adenoviral fiber with ablated

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Modified adenoviral fiber with ablated to cellular receptors by Transgene S.A. – AU 20032471

Get details on patent AU2003247128 - Modified adenoviral fiber with ablated to cellular receptors by Transgene S.A. and more at IP in Australia IP Australia AU Patents toto-ltd transformation-technologies-ltd transgene-sa AU2003247128 Modified adenoviral fiber ...

CAR- or αv integrin-binding ablated adenovirus vectors, but not fiber-modified vectors containing RGD peptide, do not change the systemic gene .

We also generated Ad vectors containing RGD peptide in the HI loop of the fiber knob. CAR-binding ablated Ad vectors mediated about 1% of gene transfer activity into CAR-positive cultured cells, ...

Selected modification of the adenovirus type 5 hexon modulates interaction with coagulation factor X and hepatocyte transduction in vivo - CO

Selected modification of the adenovirus type 5 hexon modulates interaction with coagulation factor X and hepatocyte transduction in vivo By R. Alba, Angela C. Bradshaw, Alan L ...

Effect of adenovirus serotype 5 fiber and penton modifications on in vivo tropism in rats - ScienceDire

To date, no studies have systematically assessed the impact of genetic capsid modifications on in vivo tropism in rats, ... H. Mizuguchi, et al.CAR- or αV integrin-binding ablated adenovirus vectors, but not fiber-modified vectors containing RGD peptide, do not ...

A simplified cloning strategy for the generation of an endothelial cell selective recombinant adenovirus vector — Augusta Universi

trans supply of the modified fiber. The former however, suffers from difficulties in directly manipulating large adenoviral genomic DNA. Although the latter allows easy manipulation of the small fiber gene, our studies show that the in trans supplement of the ...

Construction of Metabolically Biotinylated Adenovirus with Deleted Fiber Knob as Targeting Vector | Virology Journal | Full Te

Construction of Metabolically Biotinylated Adenovirus with Deleted Fiber Knob as Targeting Vector Yanzheng Liu 1, ... Thus, the 9RPSTCD modified fiber assembled onto hAd5 particles and was metabolically biotinylated during virus production. We then began ...

WO2004007537A3 - Modified adenoviral fiber with ablated to cellular receptors - Google Paten

The present invention concerns a modified adenoviral fiber containing at least one mutation affecting one or more amino acid residue(s) of said adenoviral fiber interacting with at least one glycosaminoglycan and/or sialic acid-containing cellular receptor, as well as a ...

Recent advances in genetic modification of adenovirus vectors for cancer treatment - Yamamoto - 2017 - Cancer Science - Wiley Online Libra

Cancer‐targeting adenovirus vectors The entry of serotype 5 adenovirus into cells requires two distinct and sequential steps. The initial step is the interaction of the fiber protein with its cellular receptor, coxsackievirus and adenovirus receptor (CAR). 4, 5 The next ...

US20060228334A1 - Modified adenoviral fiber with ablated to cellular receptors - Google Paten

The present invention concerns a modified adenoviral fiber containing at least one mutation affecting one or more amino acid residue(s) of said adenoviral fiber interacting with at least one glycosaminoglycan and/or sialic acid-containing cellular receptor, as well as a ...

Retargeting of adenoviral infection to melanoma: Combining genetic ablation of native tropism with a recombinant bispecific single‐chain diabody .

binds to both the adenoviral fiber protein and to the high molecular weight melanoma‐associated antigen (HMWMAA). ... Therefore, the development of tropism‐modified, tumor‐targeted adenoviral gene transfer vectors is a key endeavor in current gene the ...

Modified Adenoviral Vectors Ablated for Coxsackievirus–Adenovirus Receptor, αv Integrin, and Heparan Sulfate Binding Reduce In Vivo Tissue .

are the tropism determinants of adenoviral (Ad) vectors in vivo. For the development of a t... Coxsackievirus and adenovirus receptor (CAR), αv integrins, and heparan sulfate glycosaminoglycans (HSGs) are the tropism determinants of adenoviral (Ad ...

Construction of Metabolically Biotinylated Adenovirus with Deleted Fiber Knob as Targeting Vect

teins need to be modified to retarget adenoviral vector away from the liver. In the study reported here, we have ablated the native ... The presence of the modified fiber and the successful biotinylation of the fiber were confirmed using immuno-blotting analysis. As ...

Incorporation of peptides targeting EGFR and FGFR1 into the adenoviral fibre knob domain, and their evaluation as targeted cancer therapies -OR

Incorporation of peptides targeting EGFR and FGFR1 into the adenoviral fibre knob domain, and their evaluation as targeted cancer therapies Uusi-Kerttula, ...

novel method for generating and screening peptides and libraries displayed on adenovirus fiber | Nucleic Acids Research | Oxford Academ

The system is robust, allowing one to shuttle modified fiber genes into adenoviral plasmids in Escherichia coli or into replicating adenovirus genomes in mammalian cells. ...

Construction of Metabolically Biotinylated Adenovirus with Deleted Fiber Knob as Targeting Vector | SpringerLi

Figure 1 Structure, fiber trimerization and biotinylation of new 9RPSTCD adenoviral vector. (A) Structure of adenoviral vectors. All the viral vectors were replication-incompetent, E1,E3-deleted vector with a GFP as reporter gene, but each vector containing different fiber ...

MODIFIED ADENOVIRAL FIBER WITH ABLATED TO CELLULAR RECEPTORS - Patent - Europe P

The present invention concerns a modified adenoviral fiber containing at least one mutation affecting one or more amino acid residue(s) of said adenoviral fiber interacting with at least one glycosaminoglycan and/or sialic acid-containing cellular receptor, as well as a trimer of such a modified

Fiber Knob Modifications Overcome Low, Heterogeneous Expression of the Coxsackievirus-Adenovirus Receptor That Limits ...- Cancer Resear

Mutant Ads with ablated CAR binding exhibited only 5–20% of transgene expression in RMS cells seen with a wild-type vector, ... Gene transfer to RMS cells was increased >2 logs using Ads engineered with modified fiber knobs containing either an integrin The ...

Direct selection of targeted adenovirus vectors by random peptide display on the fiber knob | Gene Thera

Design of random peptide display adenovirus library constructs. (a) Schematic presentation of adenoviral cosmids and fiber-modified shuttle plasmids. A single copy of a loxP sequence substitutes for the E3 gene (76.1–84.8 m.u.). The cAd-WT has a wild-type E1 ...

CAR- or alpha v integrin-binding ablated adenovirus vectors, but not fiber-modified vectors containing RGD peptide, do not change the systemic .

Request PDF on ResearchGate | CAR- or alpha v integrin-binding ablated adenovirus vectors, but not fiber-modified vectors containing RGD peptide, do not change the systemic gene transfer properties in mice | Targeted gene delivery to the tissue of interest by ...

Short Retargeting FX-binding-ablated HAdV-5 to Communication in hexon hypervariable region 7 and the HI lo

by replacing amino acids in hexon HVR7 with RGD-4C or inserting the peptide into the fibre HI loop. These genetic modifications in the capsid were compatible with virus assembly, and could ...

Direct selection of targeted adenovirus vectors by random peptide display on the fiber knob | Gene Thera

Design of random peptide display adenovirus library constructs. (a) Schematic presentation of adenoviral cosmids and fiber-modified shuttle plasmids. A single copy of a loxP sequence substitutes for the E3 gene (76.1–84.8 m.u.). The cAd-WT has a wild-type E1 ...